pCMV3-SP-N-His (Suitable for secretary and membane protein expession)

pCMV3-SP-N-His Physical Map
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pCMV3-SP-N-His Physical Map

Comments for pCMV3-SP-N-His:

  • • CMV promoter: bases 250-837
  • • Enhancer: bases 838-1445
  • • SV40 early promoter: bases 2390-2759
  • • Hygromycin ORF: bases 2777-3802
  • • pUC origin: bases 4445-5118
  • • Kanamycin ORF: bases 5192-6007

Cloning Sites for ORF Inserting

Cloning Sites for ORF Inserting

Expression Vector pCMV3-SP-N-His Information Description

Vector Name pCMV3-SP-N-His
Vector Size 6149bp
Vector Type Mammalian Expression Vector
Expression Method Constitutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag His

pCMV3-SP-N-His is recommended for constructing the N-His tag secretary and membrane proteins expression vector which containing a naïve signal peptide. An universal signal peptide is used to instead the naïve signal peptide. 

His Tag Information

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Mammalian Expression Vectors

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