Yeast Protein Expression Service

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Yeast Protein Expression Service Introduction

Yeast protein expression system, combining with the advantages of both prokaryotic system and eukaryotic system, is a economical and high-efficiency eukaryotic protein expression system. It possesses features of low-cost media, low demands for culturing conditions and suitabilty to scale up. Compared with prokaryotic system, yeast expression system is capable of partial post-translational modification, such as glycosylation, acylation, lipidation, and phosphorylation. It is useful for to prepare high valued protein with close to natural structure.

Yeast Protein Expression Service Advantages

Over 10 years' experience of protein expression
One-stop service from gene to purified protein
Efficient expression vectors with high yield
High-throughput and large-scale protein production

Yeast Protein Expression Service Procedures

Communicate about protein expression requirements  Sign contract and make prepayment  Carry out pilot study for protein expression  Feedback pilot study result and ship sample (if feasible)  Bulk production quotation  Bulk production based on customer requirements

Yeast Protein Expression Service Items

Service procedures and items Cycle Delivery
Gene synthesis and codon optimization (optional) 1-2 weeks  
Vector construction 1-2 weeks Sequencing report
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
Vector linearization and transformation 1 weeks  
• vector linearization
• transforming linearized vector into suitable strain
• identification of positive transformants via PCR
• high-copy transformation
Expression and purification 1-2 weeks • Feasibility report
• 0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing)
• pilot scale expression, purification and condition optimization
• SDS-PAGE and UV analysis
Large-scale expression and purification (optional) 2-3 weeks • Purified protein
• SDS-PAGE report
• CoA