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TNFR2/TNFRSF1B Antibody

TNFR2/TNFRSF1B Antibody Overview

There are 20 TNFR2/TNFRSF1B antibodies which are validated in multiple tissues with various applications, including ELISA, Neutralization, FCM, ELISA(Cap), ELISA(Det), WB, IP. There are 13 TNFR2/TNFRSF1B antibody for ELISA, 2 TNFR2/TNFRSF1B antibody for Neutralization, 3 TNFR2/TNFRSF1B antibody for FCM, 1 TNFR2/TNFRSF1B antibody for ELISA(Cap), 1 TNFR2/TNFRSF1B antibody for ELISA(Det), 1 TNFR2/TNFRSF1B antibody for WB, 1 TNFR2/TNFRSF1B antibody for IP. Among all these TNFR2/TNFRSF1B antibodies, there are 2 anti-TNFR2/TNFRSF1B mouse monoclonal antibodies , 12 anti-TNFR2/TNFRSF1B rabbit monoclonal antibodies , 6 anti-TNFR2/TNFRSF1B rabbit polyclonal antibodies . All the TNFR2/TNFRSF1B anbodies are produced in house and all are in stock. TNFR2/TNFRSF1B antibody customerized service is available.

TNFR2/TNFRSF1B Antibody ELISA

TNFR2/TNFRSF1B antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TNFR2 / CD120b / TNFRSF1B (rh TNFR2 / CD120b / TNFRSF1B; Catalog#10417-H08H; NP_001057.1; Met1-Asp257). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

TNFR2/TNFRSF1B antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

TNFR2/TNFRSF1B Antibody western blot

TNFR2/TNFRSF1B Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse TNFR2 / CD120b / TNFRSF1B (rM TNFR2 / CD120b / TNFRSF1B; Catalog#50128-M08H; NP_033491.2; Met 1-Gly 258). TNFR2 / CD120b / TNFRSF1B specific IgG was purified by Mouse TNFR2 / CD120b / TNFRSF1B affinity chromatography.

TNFR2/TNFRSF1B Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

TNFR2/TNFRSF1B Antibody flow cytometry

TNFR2/TNFRSF1B Antibody flow cytometry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNFR2 / CD120b / TNFRSF1B (rM TNFR2 / CD120b / TNFRSF1B; Catalog#50128-M08H; NP_035740.2; Met1-Gly258).

TNFR2/TNFRSF1B Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

TNFR2/TNFRSF1B Antibody immunoprecipitation

TNFR2/TNFRSF1B Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Mouse TNFR2 / CD120b / TNFRSF1B (rM TNFR2 / CD120b / TNFRSF1B; Catalog#50128-M08H; NP_033491.2; Met 1-Gly 258). TNFR2 / CD120b / TNFRSF1B specific IgG was purified by Mouse TNFR2 / CD120b / TNFRSF1B affinity chromatography.

TNFR2/TNFRSF1B Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

TNFR2/TNFRSF1B Antibody Neutralization

TNFR2/TNFRSF1B Antibody Neutralization Preparation

TNFR2/TNFRSF1B Antibody Neutralization Validated

Application Neutralization
Dilution The neutralization activity of TNFR2/TNFRSF1B Neutralizing Antibody is Measured by its ability to neutralize TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 1-3 µg/mL in the presence of 1 μg/mL Recombinant Human TNFR2/TNFRSF1B, 0.25 ng/mL Recombinant Human TNFa, and 1 µg/mL actinomycin D.
Notes