PCSK9 Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Product Information
> 95 % as determined by SDS-PAGE.
< 1.0 EU per μg protein as determined by the LAL method.
0.7-1 as determined by the HABA assay.
Measured by its ability to bind recombinant LDLR-His (Cat:10231-H08H） in a functional ELISA.
A DNA sequence encoding the PCSK9 (NP_777596.2) (Met1-Gln692) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
The recombinant PCSK9 consists of 688 amino acids and predicts a molecular mass of 74.3 kDa.
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PCSK9 Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Images
PCSK9 Protein, Human, Recombinant (His & AVI Tag), Biotinylated: Alternative Names
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
proprotein convertase subtilisin/kexin type 9
Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
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