After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Быстрый заказ

Text Size:AAA

Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse SIRPA Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_007547.2
Размер кДНК:1530bp
Описание кДНК:Full length Clone DNA of Mus musculus signal-regulatory protein alpha with Flag tag.
Синоним гена:Bit, P84, SIRP, SHP-1, CD172a, Ptpns1
Виды:Mouse
переносчик:pCMV2-FLAG
Plasmid:pCMV2-mSIRPA-flag
Участок рестрикции:KpnI (two restriction sites) + XbaI (5.4kb + 0.35kb + 1.22kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutation 459 A/G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Mouse SIRPA Gene Plasmid Map
Mouse SIRPA Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged
Mouse SIRPA Gene Expression validated Image
Mouse SIRPA natural ORF mammalian expression plasmid, Flag tag
[Щелкните, чтобы увеличить изображение]
Caption:
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope.
pCMV2-FLAG Vector Information
 
Vector Name pCMV2-FLAG
Vector Size 5592bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV2-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50956-ACGRBS16760
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50956-ACRRBS16760
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50956-CFRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50956-CHRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50956-CMRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50956-CYRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин клон кДНК в вектор клонированияMG50956-GRBS5130
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50956-NFRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50956-NHRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50956-NMRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50956-NYRBS14710
Мышь SIRPA/SIRP alpha/CD172a Джин ORF экспрессии кДНК клона плазмидыMG50956-UTRBS14710
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

Ссылки
  • Timms JF. et al., 1999, Curr Biol. 9: 927-30.
  • Stofega MR. et al., 2000, J Biol Chem. 275: 28222-9.
  • Liu T. et al., 2005, J Proteome Res. 4: 2070-80.
  • Wolf-Yadlin A. et al., 2007, Proc Natl Acad Sci. 104: 5860-5.
  • Contact Us
    Изображения
    • Mouse SIRPA Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged
    • Mouse SIRPA natural ORF mammalian expression plasmid, Flag tag
      Недавно просмотренные товары
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».