Anti-TNFR2 / TNFRSF1B Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-TNFR2 / TNFRSF1B Magnetic Beads-IP Kit Product Components

Components Storage
Anti-TNFR2 / TNFRSF1B Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-TNFR2 / TNFRSF1B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-TNFR2 / TNFRSF1B Magnetic Beads-IP Kit Product Description

The Anti-TNFR2 / TNFRSF1B magnetic Beads, conjugated with Anti-TNFR2 / TNFRSF1B antibody, are used for immuneprecipitation (IP) of TNFR2 / TNFRSF1B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing TNFR2 / TNFRSF1B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound TNFR2 / TNFRSF1B proteins are dissociated from the magnetic beads using an elution buffer.

Anti-TNFR2 / TNFRSF1B Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-TNFR2 / TNFRSF1B Antibody(50128-RP02)
Immunogen
Recombinant Mouse TNFR2 / CD120b / TNFRSF1B protein (Catalog#50128-M08H)
Species Reactivity
Mouse TNFR2 / CD120b / TNFRSF1B
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse TNFR2 / CD120b / TNFRSF1B (rM TNFR2 / CD120b / TNFRSF1B; Catalog#50128-M08H; NP_033491.2; Met 1-Gly 258). TNFR2 / CD120b / TNFRSF1B specific IgG was purified by Mouse TNFR2 / CD120b / TNFRSF1B affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-TNFR2 / TNFRSF1B Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD120bALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p75ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNF-alphaR2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNF-R-IIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNF-R2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNF-R75ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFalpha-R2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFBRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Tnfr-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Tnfr2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFR80ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFRIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

TNFR2 / TNFRSF1B Background Information

Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD12b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD12b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD12b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD12b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Full Name
tumor necrosis factor receptor superfamily, member 1b
References
  • Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
  • Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
  • Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
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