IL3RA Protein

IL3RA Protein Overview

IL3RA reagents

The receptor for interleukin-3 (IL3; 147740) is a heterodimer that shares a beta chain (CSF2RB; 138981) in common with the receptors for IL5 (IL5RA; 147851) and GMCSF (CSF2RA; 306250), and, like these other cytokine receptors, has a ligand-specific alpha chain (IL3RA) (Kremer et al., 1993).

IL3RA protein family

Belongs to the type I cytokine receptor family. Type 5 subfamily.

IL3RA protein name

Recommended name
Interleukin-3 receptor subunit alpha
Short name
IL-3 receptor subunit alpha||IL-3R subunit al
Aliases
CD123
Alternative name
CD_antigen: CD123

IL3RA Gene family protein

IL3RA Protein Sequence

Species Human IL3RA protein
Length 378
Mass (Da) 43330
Sequence Human IL3RA protein sequence
Species Mouse IL3RA protein
Length 396
Mass (Da) 43195
Sequence Mouse IL3RA protein sequence
Species
Length
Mass (Da)
Sequence

IL3RA Protein Molecular Weight & PI

Interleukin-3 receptor subunit alpha precursor (IL-3 receptor subunit alpha) (IL-3R subunit alpha) (IL-3R-alpha) (IL-3RA) (CD123 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 19-378 Interleukin-3 receptor subunit alpha.

Molecular weight (Da)

41281.25

Theoretical pI

8.63

IL3RA Protein Structure

Cytokine-receptor complex
Deposited
2017-02-21   Released:  2018-02-07
Deposition Author(s)
Broughton, S.E., Parker, M.W.
Organism(s)
Homo sapiens
Expression System
Spodoptera frugiperda, Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.4000 Å
R-Value Free
0.242
R-Value Work
0.216
5UWC From PDB

Human IL3RA protein Secondary structure

IL3RA Protein Interaction

Recombinant IL3RA Protein Feature

IL3RA Protein, Human, Recombinant (His & Fc Tag)

High Purity
> 85 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA . Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind human IL3RA with a linear ranger of 0.064-1.6 μg/ml.

Recombinant IL3RA protein citations

Title
Optimization of human NK cell manufacturing: Fully-automated separation, improved <i>ex vivo </i>expansion using IL-21 with autologous feeder cells and generation of anti-CD123-CAR-expressing effector cells
Year
2017
Author
Klöß, S;Oberschmidt, O;Morgan, M;Dahlke, J;Arseniev, L;Huppert, V;Granzin, M;Gardlowski, T;Matthies, N;Soltenborn, S;Schambach, A;Koehl, U;
Journal
Hum. Gene Ther.
Title
Programmed cell death protein 1 activation preferentially inhibits CD28.CAR-T cells
Year
2018
Author
Zolov, SN;Rietberg, SP;Bonifant, CL;
Journal
Cytotherapy

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