CD16 / FCGR3 Matched ELISA Antibody Pair Set,Rhesus

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CD16 / FCGR3 Matched ELISA Antibody Pair Set,Rhesus General Information

Product name
CD16 / FCGR3 Matched ELISA Antibody Pair Set,Rhesus
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Rhesus CD16 / FCGR3
Assay range
12.5-800 pg/mL
Materials provided
1. Capture Antibody 0.07 mg/mL of mouse anti-Rhesus FCGR3 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 90013-MM05)
2. Detection Antibody 0.25 mg/mL mouse anti-Rhesus FCGR3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 90013-MM01)
3. Standard Each vial contains 31 ng of recombinant Rhesus FCGR3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.8 ng/mL is recommended.
Product overview
This CD16 / FCGR3 Matched ELISA Antibody Pair Set,Rhesus is a solid phase sandwich ELISA for quantitative determination of Rhesus CD16 / FCGR3 . It contains Rhesus CD16 / FCGR3 capture antibody, Rhesus CD16 / FCGR3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Rhesus CD16 / FCGR3 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

CD16 / FCGR3 Matched ELISA Antibody Pair Set,Rhesus Images

CD16 / FCGR3 Background Information

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Full Name
Fc receptor, IgG, low affinity III
References
  • Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
  • Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
  • Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
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