Anti-CD16 / FCGR3 Magnetic Beads Immunoprecipitation (IP) Kit

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Anti-CD16 / FCGR3 Magnetic Beads-IP Kit Product Components

Components Storage
Anti-CD16 / FCGR3 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD16 / FCGR3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-CD16 / FCGR3 Magnetic Beads-IP Kit Product Description

The Anti-CD16 / FCGR3 magnetic Beads, conjugated with Anti-CD16 / FCGR3 antibody, are used for immuneprecipitation (IP) of CD16 / FCGR3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD16 / FCGR3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD16 / FCGR3 proteins are dissociated from the magnetic beads using an elution buffer.

Anti-CD16 / FCGR3 Magnetic Beads-IP Kit Antibody Information

Antibody
Anti-CD16 / FCGR3 Antibody(50326-R015)
Immunogen
Recombinant Mouse CD16 / FCGR3 protein (Catalog#50326-M08H)
Species Reactivity
Mouse CD16 / FCGR3
Source
Monoclonal Mouse Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16 / FCGR3 (rM CD16 / FCGR3; Catalog#50326-M08H; P08508-1; Met 1-Thr 215).
Applications
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD16 / FCGR3 Magnetic Beads Immunoprecipitation (IP) Kit: Alternative Names

Anti-CD16ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD16 / FCGR3 Background Information

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Full Name
Fc receptor, IgG, low affinity III
References
  • Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
  • Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
  • Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
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