Anti-c-Kit Antibody (FITC)

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Anti-c-Kit Antibody (FITC) (Mouse Monoclonal antibody) General Information

Product name
Anti-c-Kit Antibody (FITC)
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human c-Kit
Immunogen
Recombinant Human c-Kit Protein (Catalog#11996-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human c-Kit (rh c-Kit; Catalog#11996-H08H; P10721-2; Met1-Thr516) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG1 Clone #41
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.03%ProClin300
Conjugate
FITC
Concentration
5 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !

Anti-c-Kit Antibody (FITC) (Mouse Monoclonal antibody) Images

Flow cytometric analysis of Human KIT(CD117) expression on HEL92 cells. Cells were stained with FITC-conjugated anti-Human KIT(CD117). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-c-Kit Antibody (FITC): Alternative Names

Anti-C-Kit Antibody; Anti-CD117 Antibody; Anti-PBT Antibody; Anti-SCFR Antibody

c-Kit Background Information

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.
Full Name
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
References
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.

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