Anti-UCHL3 Antibody

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Anti-UCHL3 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-UCHL3 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Rat
Specificity
Rat UCHL3
Has cross-reactivity in ELISA with
Human UCHL3
Mouse UCHL3
Immunogen
Recombinant Rat UCHL3 protein (Catalog#80087-R07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Rat UCHL3 (rR UCHL3; Catalog#80087-R07E; Q91Y78; Glu2-Ala230).
Source
Monoclonal Rabbit IgG Clone #007
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-UCHL3 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-UCHL3 Antibody (Rabbit Monoclonal antibody) Images

Immunochemical staining of rat UCHL3 in rat testis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:31 kDa

rat UCHL3 was immunoprecipitated using:

Lane A:0.5 mg k562 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

Lane C:0.5 mg SH-SY5Y Whole Cell Lysate

2 µL anti-rat UCHL3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-rat UCHL3 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 26 kDa

Observed band size: 26 kDa

UCHL3 Background Information

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to thepeptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
Full Name
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
References
  • Wood,M.A. et al., 2005, Hippocampus  15 (5):610-21.
  • Kwon,J. et al., 2006, Exp Anim  55 (1):35-43.
  • Setsuie,R. et al., 2009, Neurochem Int  54 (5-6):314-21.
  • Setsuie,R. et al., 2010, Neurochem Int  56 (8):911-8.
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