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Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat TPST1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001011903.2
Размер кДНК:1113bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus tyrosylprotein sulfotransferase 1 with N terminal HA tag.
Синоним гена:Tpst1
Виды:Rat
переносчик:pCMV3-SP-N-HA
Plasmid:
Участок рестрикции:
Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка on other vectors
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG80470-ACGRBS15396
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG80470-ACRRBS15396
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG80470-CFRBS13343
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG80470-CHRBS13343
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG80470-CMRBS13343
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG80470-CYRBS13343
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG80470-NFRBS13343
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG80470-NHRBS13343
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG80470-NMRBS13343
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG80470-NYRBS13343
Крыса TPST1 Джин клон кДНК в вектор клонированияRG80470-URBS5132
Крыса TPST1 Джин ORF экспрессии кДНК клона плазмидыRG80470-UTRBS13343
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Фон

Protein-tyrosine sulfotransferase 1, also known as Tyrosylprotein sulfotransferase 1 and TPST1, is a single-pass type I I membrane protein which belongs to the protein sulfotransferase family. Tyrosine O-sulfation is a common posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called tyrosylprotein sulfotransferase (TPST) that catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to tyrosine residues within acidic motifs of polypeptides. Tyrosine O-sulfation has been shown to be important in protein-protein interactions in several systems. Tyrosine sulfation is mediated by one of two Golgi isoenzymes, called tyrosylprotein sulfotransferases (TPST-1 and TPST-2). A relatively small number of proteins are known to undergo tyrosine sulfation, including certain adhesion molecules, G-protein-coupled receptors, coagulation factors, serpins, extracellular matrix proteins, and hormones. TPST1 is a human tyrosylprotein sulfotransferase that uses 3'phosphoadenosine-5'phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. TPST1 bears N-linked glycosyl residues exclusively at position Asn60 and Asn262. TPST1 and TPST2 have distinct biological roles that may reflect differences in their macromolecular substrate specificity.

Ссылки
  • Ouyang Y.-B.et al., 1998, Proc. Natl. Acad. Sci. USA. 95: 2896-901.
  • Ouyang,Y.B. et al., 2002,J Biol Chem. 277 (26):23781-7.
  • Hoffhines, A.J. et al., 2006, J Biol Chem. 281 (49):37877-87.
  • Goettsch,S. et al., 2006, J Mol Biol. 361 (3):436-49.
  • Westmuckett, A.D. et al., 2008, Gen Comp Endocrinol. 156 (1):145-53.
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    Каталог: RG80470-NY
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    Наличие2-3 weeks
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