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Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat SPN Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001271086.1
Размер кДНК:1173bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus persephin with N terminal HA tag.
Синоним гена:PSP, PspnS
Виды:Rat
переносчик:pCMV3-SP-N-HA
Plasmid:
Участок рестрикции:
Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка on other vectors
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG80432-ACGRBS15400
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG80432-ACRRBS15400
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG80432-CFRBS13340
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG80432-CHRBS13340
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG80432-CMRBS13340
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG80432-CYRBS13340
Крыса SPN/CD43 Джин клон кДНК в вектор клонированияRG80432-GRBS5130
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG80432-NFRBS13340
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG80432-NHRBS13340
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG80432-NMRBS13340
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG80432-NYRBS13340
Крыса SPN/CD43 Джин ORF экспрессии кДНК клона плазмидыRG80432-UTRBS13340
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Фон

CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals; it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. In addition, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.

Ссылки
  • . Layseca-Espinosa E, et al. (2003) Journal of Leukocyte Biology. 74(6): 1083-93.
  • Cannon JL, et al. (2011) Mol Biol Cell. 22(7):954-63.
  • Pallant A,et al. (1989). Proc Natl Acad Sci. 86 (4): 1328–32.
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    Каталог: RG80432-NY
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    Наличие2-3 weeks
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