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Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat PTN Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_017066.2
Размер кДНК:507bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus pleiotrophin with C terminal Myc tag.
Синоним гена:Hbnf
Виды:Rat
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG81121-ACGRBS15396
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG81121-ACRRBS15396
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG81121-CFRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG81121-CHRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG81121-CMRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG81121-CYRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG81121-NFRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG81121-NHRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG81121-NMRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG81121-NYRBS13343
Крыса Pleiotrophin / PTN / HB-GAM Джин клон кДНК в вектор клонированияRG81121-URBS5132
Крыса Pleiotrophin / PTN / HB-GAM Джин ORF экспрессии кДНК клона плазмидыRG81121-UTRBS13343
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Фон

HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

Ссылки
  • Vanderwinden JM, et al. (1992) Cellular distribution of the new growth factor pleiotrophin (HB-GAM) mRNA in developing and adult rat tissues. Anat Embryol. 186(4):387-406.
  • Lauri SE, et al. (1996) Activity-induced enhancement of HB-GAM expression in rat hippocampal slices. Neuroreport. 7(10):1670-4.
  • Pavlov I, et al. (2002) Role of heparin-binding growth-associated molecule (HB-GAM) in hippocampal LTP and spatial learning revealed by studies on overexpressing and knockout mice. Mol Cell Neurosci. 20(2):330-42.
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    Каталог: RG81121-CM
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    Наличие2-3 weeks
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