After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Быстрый заказ

Text Size:AAA

Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat PDPN Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_019358.1
Размер кДНК:501bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus podoplanin with C terminal Flag tag.
Синоним гена:E11, Gp38, OTS-8, RTI40, T1-alpha
Виды:Rat
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG81154-ACGRBS15400
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG81154-ACRRBS15400
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG81154-CFRBS13340
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG81154-CHRBS13340
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG81154-CMRBS13340
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG81154-CYRBS13340
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG81154-NFRBS13340
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG81154-NHRBS13340
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG81154-NMRBS13340
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG81154-NYRBS13340
Крыса Podoplanin Джин клон кДНК в вектор клонированияRG81154-URBS5130
Крыса Podoplanin Джин ORF экспрессии кДНК клона плазмидыRG81154-UTRBS13340
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.

Ссылки
  • Kimura, N. et al., 2005,  Pathol Int. 55 (2): 83-86.
  • Ordóñez, N.G., 2006, Adv Anat Pathol. 13 (2): 83-88.
  • Wicki, A. et al., 2007,  Br. J. Cancer. 96 (1): 1-5.
  • Fujii,T. et al., 2008, Mod Pathol. 21 (2): 125-130.
  • Sawa,Y. et al., 2008, Acta Histochem Cytochem. 41 (5):121-126.
  • Kaddu, S. et al., 2009, Am J Dermatopathol.  31 (2): 137-139.
  • All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».