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Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat IL18R1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001106905.2
Размер кДНК:1614bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus interleukin 18 receptor 1 with N terminal Flag tag.
Синоним гена:Il18r1, Il18r1_predicted
Виды:Rat
переносчик:pCMV3-SP-N-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG80191-ACGRBS16760
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG80191-ACRRBS16760
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG80191-CFRBS14710
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG80191-CHRBS14710
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG80191-CMRBS14710
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG80191-CYRBS14710
Крыса IL18R1 Джин клон кДНК в вектор клонированияRG80191-GRBS5130
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG80191-NFRBS14710
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG80191-NHRBS14710
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG80191-NMRBS14710
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG80191-NYRBS14710
Крыса IL18R1 Джин ORF экспрессии кДНК клона плазмидыRG80191-UTRBS14710
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Product nameProduct name
Фон

Interleukin-18 receptor 1 (IL18R1) also known as CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein and CD218a, is an interleukin receptor of the immunoglobulin superfamily. IL18R1 is found expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression is found in Hodgkin disease cell lines. This receptor is specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IL18R1 contains 3 Ig-like C2-type (immunoglobulin-like) domains and 1 TIR domain. It is a single-pass type I membrane protein. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The increased expression of IL18R1 may contribute pathogenically to disease and is therefore a potential therapeutic target. The absence of a genetic association in the IL18R1 gene itself suggests regulation from other parts of the genome, or as part of the inflammatory cascade in multiple sclerosis without a prime genetic cause.

Ссылки
  • Nadif R, et al.. (2006) IL18 and IL18R1 polymorphisms, lung CT and fibrosis: A longitudinal study in coal miners. Eur Respir J. 28(6): 1100-5.
  • Haralambieva IH, et al.. (2011) Common SNPs/haplotypes in IL18R1 and IL18 genes are associated with variations in hum oral immunity to smallpox vaccination in Caucasians and African Americans. J Infect Dis. 204(3): 433-41.
  • Hulin-Curtis SL, et al.. (2012) Evaluation of IL18 and IL18R1 polymorphisms: genetic susceptibility to knee osteoarthritis. Int J Immunogenet. 39(2): 106-9.
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    Каталог: RG80191-NF
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