|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL11RA ORF mammalian expression plasmid, C-GFPSpark tag||RG80179-ACG|
|Rat IL11RA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80179-ACR|
|Rat IL11RA ORF mammalian expression plasmid, C-Flag tag||RG80179-CF|
|Rat IL11RA ORF mammalian expression plasmid, C-His tag||RG80179-CH|
|Rat IL11RA ORF mammalian expression plasmid, C-Myc tag||RG80179-CM|
|Rat IL11RA ORF mammalian expression plasmid, C-HA tag||RG80179-CY|
|Rat IL11RA Gene cDNA clone plasmid||RG80179-G|
|Rat IL11RA ORF mammalian expression plasmid, N-Flag tag||RG80179-NF|
|Rat IL11RA ORF mammalian expression plasmid, N-His tag||RG80179-NH|
|Rat IL11RA ORF mammalian expression plasmid, N-Myc tag||RG80179-NM|
|Rat IL11RA ORF mammalian expression plasmid, N-HA tag||RG80179-NY|
|Rat IL11RA natural ORF mammalian expression plasmid||RG80179-UT|
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Interleukin 11 receptor, alpha subunit (IL11RA/IL-11RA) is a subunit of the interleukin 11 receptor which is a member of the hematopoietic cytokine receptor family. IL11RA/IL-11RA is expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. It is also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Alternative splicing has been observed at this locus, and three variants encoding two different isoforms have been identified. IL11RA/IL-11RA is a receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. Defects in IL11RA/IL-11RA are a cause of craniosynostosis and dental anomalies (CRSDA). CRSDA is a disorder characterized by craniosynostosis, maxillary hypoplasia, and dental anomalies, including malocclusion, delayed and ectopic tooth eruption, and/or supernumerary teeth. Some patients also display minor digit anomalies, such as syndactyly and/or clinodactyly.