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Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat FGF9 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_012952.1
Размер кДНК:627bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus fibroblast growth factor 9 with N terminal Flag tag.
Синоним гена:Fgf9
Виды:Rat
переносчик:pCMV3-SP-N-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG80143-ACGRBS15396
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG80143-ACRRBS15400
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG80143-CFRBS13343
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG80143-CHRBS13343
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG80143-CMRBS13343
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG80143-CYRBS13343
Крыса FGF9 Джин клон кДНК в вектор клонированияRG80143-GRBS5130
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG80143-NFRBS13343
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG80143-NHRBS13340
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG80143-NMRBS13340
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG80143-NYRBS13340
Крыса FGF9 Джин ORF экспрессии кДНК клона плазмидыRG80143-UTRBS13343
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Фон

Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. FGF9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

Ссылки
  • Giri D, et al. (1999) FGF9 is an autocrine and paracrine prostatic growth factor expressed by prostatic stromal cells. J Cell Physiol. 180(1): 53-60.
  • Schmahl J, et al. (2004) Fgf9 induces proliferation and nuclear localization of FGFR2 in Sertoli precursors during male sex determination. Development. 131(15): 3627-36.
  • Garcès A, et al. (2000) FGF9: a motoneuron survival factor expressed by medial thoracic and sacral motoneurons. J Neurosci Res. 60(1): 1-9.
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    Каталог: RG80143-NF
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    Наличие2-3 weeks
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