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Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-His Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat CD3D Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_013169.1
Размер кДНК:522bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus CD3 molecule, delta with C terminal His tag.
Синоним гена:Cd3d
Виды:Rat
переносчик:pCMV3-C-His
Plasmid:
Участок рестрикции:
Последовательность меток:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-His Метка on other vectors
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG80313-ACGRBS15400
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG80313-ACRRBS15400
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG80313-CFRBS13340
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG80313-CHRBS13340
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG80313-CMRBS13340
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG80313-CYRBS13340
Крыса CD3d/CD3 delta Джин клон кДНК в вектор клонированияRG80313-GRBS5130
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG80313-NFRBS13340
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG80313-NHRBS13340
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG80313-NMRBS13340
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG80313-NYRBS13340
Крыса CD3d/CD3 delta Джин ORF экспрессии кДНК клона плазмидыRG80313-UTRBS13340
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Фон

T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCRalpha and beta chains along with invariant CD3gamma, delta, epsilon, and zeta chains. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCRgamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.

Ссылки
  • Roifman CM. (2004) CD3 delta immunodeficiency. Curr Opin Allergy Clin Immunol. 4(6): 479-84.
  • Pan Q, et al. (2006) Different role for mouse and human CD3delta/epsilon heterodimer in preT cell receptor (preTCR) function: human CD3delta/epsilon heterodimer restores the defective preTCR function in CD3gamma- and CD3gammadelta-deficient mice. Mol Immunol. 43(11): 1741-50.
  • Le Deist F, et al. (2007) Expression anomalies of the CD3-TCR complex expression and immunodeficiencies. Med Sci (Paris). 23(2): 161-6.
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    Каталог: RG80313-CH
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    Наличие2-3 weeks
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