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Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat CALR Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_022399.2
Размер кДНК:1251bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus calreticulin with C terminal Flag tag.
Синоним гена:Calr
Виды:Rat
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG81126-ACGRBS15400
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG81126-ACRRBS15400
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG81126-CFRBS13340
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG81126-CHRBS13340
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG81126-CMRBS13340
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG81126-CYRBS13340
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG81126-NFRBS13340
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG81126-NHRBS13340
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG81126-NMRBS13340
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG81126-NYRBS13340
Крыса Calreticulin Джин клон кДНК в вектор клонированияRG81126-URBS5130
Крыса Calreticulin Джин ORF экспрессии кДНК клона плазмидыRG81126-UTRBS13340
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Фон

Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.

Ссылки
  • Michalak M, et al. (2002) Calreticulin in cardiac development and pathology. Biochim Biophys Acta. 1600(1-2):32-7.
  • Chao MP, et al. (2010) Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2(63):63ra94.
  • Andrin, C, et al. (1998) Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules. Biochemistry. 37(29):10386-94.
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    Каталог: RG81126-CF
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    Количество:+-
    Наличие2-3 weeks
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