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Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rat APOH Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001009626.1
Размер кДНК:1038bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus apolipoprotein H (beta-2-glycoprotein I) with N terminal Myc tag.
Синоним гена:Apoh
Виды:Rat
переносчик:pCMV3-SP-N-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка on other vectors
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG80701-ACGRBS15396
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG80701-ACRRBS15396
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG80701-CFRBS13343
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG80701-CHRBS13343
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG80701-CMRBS13343
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG80701-CYRBS13343
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG80701-NFRBS13343
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG80701-NHRBS13343
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG80701-NMRBS13343
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG80701-NYRBS13343
Крыса Apolipoprotein H/APOH Джин клон кДНК в вектор клонированияRG80701-URBS5132
Крыса Apolipoprotein H/APOH Джин ORF экспрессии кДНК клона плазмидыRG80701-UTRBS13343
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Фон

Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. APOH appears to completely inhibit serotonin release by the platelets and prevents subsequent waves of the ADP-induced aggregation. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH).

Ссылки
  • Kamboh MI, et al. (1998) Genetics of apolipoprotein H (beta2-glycoprotein I) and anionic phospholipid binding. Lupus. 7 Suppl 2: S10-3.
  • Singh P, et al. (2002) Genetics of apolipoprotein H (beta2-glycoprotein I) polymorphism in India. Ann Hum Biol. 29(3): 247-55.
  • Xia J, et al. (2004) Apolipoprotein H gene polymorphisms and risk of primary cerebral hemorrhage in a Chinese population. Cerebrovasc Dis. 17(2-3): 197-203.
  • Chen Q, et al. (2006) Complete DNA sequence variation in the apolipoprotein H (beta-glycoprotein I) gene and identification of informative SNPs. Ann Hum Genet. 70(Pt 1): 1-11.
  • Leduc MS, et al. (2008) Comprehensive evaluation of apolipoprotein H gene (APOH) variation identifies novel associations with measures of lipid metabolism in GENOA. J Lipid Res. 49(12): 2648-56.
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    Каталог: RG80701-NM
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