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Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rabbit IL1B Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001082201.1
Размер кДНК:807bp
Описание кДНК:Full length Clone DNA of Rabbit interleukin 1, beta with N terminal Flag tag.
Синоним гена:IL1-BETA, IL1B
Виды:Rabbit
переносчик:pCMV3-SP-N-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаTG65004-ACGRBS15400
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаTG65004-ACRRBS15400
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаTG65004-CFRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаTG65004-CHRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаTG65004-CMRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаTG65004-CYRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин клон кДНК в вектор клонированияTG65004-GRBS5130
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаTG65004-NFRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаTG65004-NHRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаTG65004-NMRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаTG65004-NYRBS13340
Кролик IL1B/IL-1B/IL-1 beta Джин ORF экспрессии кДНК клона плазмидыTG65004-UTRBS13340
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).

Ссылки
  • Kim SH, et al. (2008) Association of -31TC and -511 CT polymorphisms in the interleukin 1 beta (IL1B) promoter in Korean keratoconus patients. Mol Vis. 14:2109-16.
  • Wang ZC, et al. (2002) T helper 1-type immunity to trophoblast antigens in women with a history of recurrent pregnancy loss is associated with polymorphism of the IL1B promoter region. Genes Immun. 3(1): 38-42.
  • Mattuzzi S, et al. (2007) Association of polymorphisms in the IL1B and IL2 genes with susceptibility and severity of systemic sclerosis. J Rheumatol. 34(5): 997-1004.
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    Каталог: TG65004-NF
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    Наличие2-3 weeks
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