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Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Rabbit CD3E Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001082001.1
Размер кДНК:606bp
Описание кДНК:Full length Clone DNA of Rabbit CD3e molecule, epsilon (CD3-TCR complex) with N terminal Flag tag.
Синоним гена:CD3E
Виды:Rabbit
переносчик:pCMV3-SP-N-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаTG65002-ACGRBS15400
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаTG65002-ACRRBS15400
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаTG65002-CFRBS13340
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаTG65002-CHRBS13340
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаTG65002-CMRBS13340
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаTG65002-CYRBS13340
Кролик CD3e/CD3 epsilon Джин клон кДНК в вектор клонированияTG65002-GRBS5130
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаTG65002-NFRBS13340
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаTG65002-NHRBS13340
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаTG65002-NMRBS13340
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаTG65002-NYRBS13340
Кролик CD3e/CD3 epsilon Джин ORF экспрессии кДНК клона плазмидыTG65002-UTRBS13340
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Фон

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

Ссылки
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.
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    Каталог: TG65002-NF
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