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Человек RAET1L Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Human RAET1L Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_130900.2
Размер кДНК:741bp
Описание кДНК:Full length Clone DNA of Homo sapiens retinoic acid early transcript 1L.
Синоним гена:ULBP6
Виды:Human
переносчик:pGEM-T Vector
Plasmid:pGEM-RAET1L
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutation 254 T/C resulting in the amino acid Met substitution by Thr.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Человек RAET1L Джин клон кДНК в вектор клонирования on other vectors
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