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Крыса PTGDS / L-PGDS Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Rat PTGDS Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_013015.2
Размер кДНК:570bp
Описание кДНК:Full length Clone DNA of Rattus norvegicus prostaglandin D2 synthase (brain).
Синоним гена:PH2DISO
Виды:Rat
переносчик:pGEM-T Vector
Plasmid:pGEM-ratPtgds
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Крыса PTGDS / L-PGDS Джин клон кДНК в вектор клонирования on other vectors
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаRG81340-ACGRBS15400
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаRG81340-ACRRBS15400
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаRG81340-CFRBS13340
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаRG81340-CHRBS13340
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаRG81340-CMRBS13340
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаRG81340-CYRBS13340
Крыса PTGDS / L-PGDS Джин клон кДНК в вектор клонированияRG81340-GRBS5130
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаRG81340-NFRBS13340
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаRG81340-NHRBS13340
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаRG81340-NMRBS13340
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаRG81340-NYRBS13340
Крыса PTGDS / L-PGDS Джин ORF экспрессии кДНК клона плазмидыRG81340-UTRBS13340
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Фон

PTGDS, also known as L-PGDS, belongs to the calycin superfamily,lipocalin family. Lipocalins share limited regions of sequence homology and a common tertiary structure architecture. They transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. PTGDS is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of PGH2 to PGD2. It is involved in smooth muscle contraction/relaxation and a variety of central nervous system functions. PTGDS may have an anti-apoptotic role in oligodendrocytes. It binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.

Ссылки
  • Aebersold R, et al. (1993) Identification of a brain-specific human cerebrospinal fluid glycoprotein, beta-trace protein. Theor Electrophor. 3:229-234.
  • Oliver K, et al. (2004) DNA sequence and analysis of human chromosome 9. Nature. 429:369-374.
  • Bonaldo MF, et al. (1997) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 6(9):791-806.
  • Size / Price
    Каталог: RG81340-G
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    Наличие2-3 weeks
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