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Человек PCSK9/NARC1 Джин клон кДНК в вектор клонирования

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Human PCSK9 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_174936.3
Размер кДНК:2079bp
Описание кДНК:Full length Clone DNA of Homo sapiens proprotein convertase subtilisin/kexin type 9.
Синоним гена:FH3, PC9, NARC1, LDLCQ1, NARC-1, HCHOLA3
Виды:Human
переносчик:pGEM-T Vector
Plasmid:pGEM-PCSK9
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1420 G/A, 2009 G/A resulting in the amino acid Val substitution by Ile, Gly substitution by Glu and 1026 A/G,1380 A/G not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Человек PCSK9/NARC1 Джин клон кДНК в вектор клонирования on other vectors
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG15813-ACGRBS16760
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG15813-ACRRBS16760
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG15813-CFRBS14710
Человек PCSK9 ORF mammalian expression plasmid, C-His МеткаHG15813-CHRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG15813-CMRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG15813-CYRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG15813-G-HRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG15813-NFRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG15813-NHRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG15813-NMRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG15813-NYRBS14710
Человек PCSK9/NARC1 Джин ORF экспрессии кДНК клона плазмидыHG15813-UTRBS14710
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Фон

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

References

Ссылки
  • Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
  • Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
  • Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
  • Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
  • Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
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    Каталог: HG15813-G
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