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Человек PARK7/DJ-1 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Human PARK7 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001123377.1
Размер кДНК:570bp
Описание кДНК:Full length Clone DNA of Homo sapiens parkinson protein 7.
Синоним гена:DJ1, DJ-1, HEL-S-67p
Виды:Human
переносчик:pGEM-T Vector
Plasmid:pGEM-hPARK7
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Человек PARK7/DJ-1 Джин клон кДНК в вектор клонирования on other vectors
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG16199-ACGRBS15396
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG16199-ACRRBS15396
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаHG16199-ANGRBS15396
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаHG16199-ANRRBS15396
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG16199-CFRBS13343
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG16199-CHRBS13343
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG16199-CMRBS13343
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG16199-CYRBS13343
Человек PARK7/DJ-1 Джин клон кДНК в вектор клонированияHG16199-GRBS5132
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG16199-NFRBS13343
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG16199-NHRBS13343
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG16199-NMRBS13343
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG16199-NYRBS13343
Человек PARK7/DJ-1 Джин ORF экспрессии кДНК клона плазмидыHG16199-UTRBS13343
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Фон

Parkinson's disease locus DJ-1 (PARK7) is a differentially expressed transcript. DJ-1 plays a physiologic role in protection of erythroid cells from oxidant damage, a function unmasked in the context of oxidative stress. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (PD). DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). DJ-1 regulates redox signaling kinase pathways and acts as a transcriptional regulator of antioxidative gene batteries. Therefore, DJ-1 is an important redox-reactive signaling intermediate controlling oxidative stress after ischemia, upon neuroinflammation, and during age-related neurodegenerative processes. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke.

Ссылки
  • Takahashi K, et al. (2001). DJ-1 positively regulates the androgen receptor by impairing the binding of PIASx alpha to the receptor. J. Biol. Chem. (United States). 276 (40): 37556-63.
  • Niki, Takeshi, et al. (2003). DJBP: a novel DJ-1-binding protein, negatively regulates the androgen receptor by recruiting histone deacetylase complex, and DJ-1 antagonizes this inhibition by abrogation of this complex. Mol. Cancer Res. (United States). 1 (4): 247-61.
  • Kahle PJ, et al. (2009) DJ-1 and prevention of oxidative stress in Parkinson's disease and other age-related disorders. Free Radic Biol Med. 47(10): 1354-61.
  • Xu X, et al. (2010) The familial Parkinson's disease gene DJ-1 (PARK7) is expressed in red cells and plays a role in protection against oxidative damage. Blood Cells Mol Dis. 45(3): 227-32.
  • Marcondes AM, et al. (2010) Identification of DJ-1/PARK-7 as a determinant of stroma-dependent and TNF-alpha-induced apoptosis in MDS using mass spectrometry and phosphopeptide analysis. Blood. 115(10): 1993-2002.
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    Каталог: HG16199-G
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