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Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse NTRK3 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_008746.5
Размер кДНК:2478bp
Описание кДНК:Full length Clone DNA of Mus musculus neurotrophic tyrosine kinase, receptor, type 3 with C terminal Myc tag.
Синоним гена:TrkC, AW125844
Виды:Mouse
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50320-ACGRBS16760
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50320-ACRRBS16760
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50320-CFRBS14710
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50320-CHRBS14710
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50320-CMRBS14710
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50320-CYRBS14710
Мышь TrkC/NTRK3 Джин клон кДНК в вектор клонированияMG50320-GRBS5130
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50320-NFRBS14710
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50320-NHRBS14710
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50320-NMRBS14710
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50320-NYRBS14710
Мышь TrkC/NTRK3 Джин ORF экспрессии кДНК клона плазмидыMG50320-UTRBS14710
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Фон

NT-3 growth factor receptor also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudatoputamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. In the PNS, trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.

Ссылки
  • Tessarollo L, et al. (1993) trkC, a receptor for neurotrophin-3, is widely expressed in the developing nervous system and in non-neuronal tissues. Development. 118(2): 463-75.
  • Lamballe F, et al. (1994) Developmental expression of trkC, the neurotrophin-3 receptor, in the mammalian nervous system. J Neurosci. 14(1): 14-28.
  • Klein R, et al. (1994) Disruption of the neurotrophin-3 receptor gene trkC eliminates la muscle afferents and results in abnormal movements. Nature. 368(6468): 249-51.
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