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Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse TNF Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_013693.2
Размер кДНК:708bp
Описание кДНК:Full length Clone DNA of Mus musculus tumor necrosis factor with C terminal Myc tag.
Синоним гена:DIF, Tnfa, TNFSF2, Tnfsf1a, TNFalpha, MGC151434, TNF-alpha, Tnf
Виды:Mouse
переносчик:pCMV3-C-Myc
Plasmid:pCMV3-mTNF-Myc
Участок рестрикции:KpnI + XbaI (6kb + 0.75kb)
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Mouse TNF Gene Plasmid Map
Mouse TNFα natural ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50349-ACGRBS15400
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50349-ACRRBS15400
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50349-CFRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50349-CHRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50349-CMRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50349-CYRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин клон кДНК в вектор клонированияMG50349-MRBS5130
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50349-NFRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50349-NHRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50349-NMRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50349-NYRBS13340
Мышь TNF-alpha/TNFA/TNFSF2 Джин ORF экспрессии кДНК клона плазмидыMG50349-UTRBS13340
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Product nameProduct name
Фон

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Ссылки
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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