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Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse TNFRSF1A Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_011609.4
Размер кДНК:1365bp
Описание кДНК:Full length Clone DNA of Mus musculus tumor necrosis factor receptor superfamily, member 1a with C terminal HA tag.
Синоним гена:FPF, p55, TNF-R, TNFAR, TNFRI, Tnfr1, p55-R, CD120a, TNF-R1, TNFR60, Tnfr-2, TNF-R-I, TNF-R55, TNFRp55, TNF-alphaR1
Виды:Mouse
переносчик:pCMV3-C-HA
Plasmid:
Участок рестрикции:
Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-HA Метка on other vectors
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50496-ACGRBS15400
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50496-ACRRBS15400
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50496-CFRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50496-CHRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50496-CMRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50496-CYRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин клон кДНК в вектор клонированияMG50496-MRBS5130
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50496-NFRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50496-NHRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50496-NMRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50496-NYRBS13340
Мышь TNFR1/TNFRSF1A/CD120a Джин ORF экспрессии кДНК клона плазмидыMG50496-UTRBS13340
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Фон

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Ссылки
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.
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    Каталог: MG50496-CY
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