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Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка

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    Мышь TP53 Информация о продукте «Клон cDNA»
    Gene_bank_ref_id:NM_011640.3
    Размер кДНК:1173bp
    Описание кДНК:Full length Clone DNA of Mus musculus transformation related protein 53 with N terminal HA tag.
    Синоним гена:bbl, bfy, bhy, p53, Tp53, Trp53
    Виды:Mouse
    переносчик:pCMV3-N-HA
    Plasmid:
    Участок рестрикции:
    Последовательность меток:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    Описание последовательности:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with TP53 qPCR primers for gene expression analysis, MP200526 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Склад:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-HA Метка on other vectors
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50534-ACGRBS15400
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50534-ACRRBS15400
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаMG50534-ANGRBS15400
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаMG50534-ANRRBS15400
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50534-CFRBS13340
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50534-CHRBS13340
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50534-CMRBS13340
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50534-CYRBS13340
    Мышь p53 Джин клон кДНК в вектор клонированияMG50534-MRBS5130
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50534-NFRBS13340
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50534-NHRBS13340
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50534-NMRBS13340
    Мышь p53 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50534-NYRBS13340
    Мышь p53 Джин ORF экспрессии кДНК клона плазмидыMG50534-UTRBS13340
     Узнайте больше о векторов экспрессии,
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    Фон

    p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitn ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    Ссылки
  • Bakhrat A, et al. (2010) Drosophila Chk2 and p53 proteins induce stage-specific cell death independently during oogenesis. Apoptosis. 15(12):1425-34.
  • Kurzhals RL, et al. (2011) Chk2 and p53 are haploinsufficient with dependent and independent functions to eliminate cells after telomere loss. PLoS Genet. 7(6):e1002103.
  • Pardi N, et al. (2011) In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53. Acta Biol Hung. 62(4):397-412.
  • Wells BS, et al. (2012) Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53. Dev Biol. 361(2):263-76.
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    Каталог: MG50534-NY
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