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Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse MAP3K8 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_007746.2
Размер кДНК:1443 bp
Описание кДНК:Full length Clone DNA of Mus musculus mitogen-activated protein kinase kinase kinase 8 with N terminal Flag tag.
Синоним гена:c-COT,Cot,Cot/Tpl2,Est,Estf,Tpl-2,Tpl2
Виды:Mouse
переносчик:pCMV3-N-FLAG
Plasmid:pCMV3-Flag-mMAP3K8
Участок рестрикции:KpnI + XbaI(6kb+1.44kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at ambient temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50438-ACGRBS15400
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50438-ACRRBS15400
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаMG50438-ANGRBS15400
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаMG50438-ANRRBS15400
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50438-CFRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50438-CHRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50438-CMRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50438-CYRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин клон кДНК в вектор клонированияMG50438-MRBS5130
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50438-NFRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50438-NHRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50438-NMRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50438-NYRBS13340
Мышь TPL2/MAP3K8/MEKK8 Джин ORF экспрессии кДНК клона плазмидыMG50438-UTRBS13340
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Фон

Mitogen-activated protein kinase kinase kinase 8, also known as Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2 and MAP3K8, is a cytoplasm protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K8 is expressed in several normal tissues and human tumor-derived cell lines. Isoform 1 of MAP3K8 is activated specifically during the S and G2/M phases of the cell cycle. MAP3K8 is required for TLR4 activation of the MEK/ERK pathway. It is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. MAP3K8 plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. MAP3K8 oncogene linked to human endometrial carcinoma suggesting that it may be another molecule involved in human endometrial cancer. MAP3K8 may also be an important mediator of intracellular mechanotransduction in human bone marrow-derived mesenchymal stem cells (MSCs).

Ссылки
  • Clark,A.M. et al., 2004, Genes Chromosomes Cancer. 41 (2):99-108.
  • Chan,H. et al., 2005, Biochem Biophys Res Commun. 328 (1):198-205.
  • Aparecida Alves,C. et al., 2006, Eur J Gynaecol Oncol. 27 (6):589-93.
  • Mielke,L.A. et al., 2009, J Immunol. 183 (12):7984-93.
  • Glossop,J.R. et al., 2009,Gene Expr Patterns  9 (5):381-8. 
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