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Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse LAMP3 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_177356.3
Размер кДНК:1236bp
Описание кДНК:Full length Clone DNA of Mus musculus lysosomal-associated membrane protein 3 with N terminal Myc tag.
Синоним гена:LAMP, Cd208, DCLAMP, TSC403, DC-LAMP, 1200002D17Rik, Lamp3
Виды:Mouse
переносчик:pCMV3-SP-N-Myc
Plasmid:pCMV3-Myc-mLAMP3
Участок рестрикции:KpnI + XbaI (6kb + 1.33kb)
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Mouse LAMP3 Gene Plasmid Map
Mouse LAMP3 natural ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка on other vectors
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50785-ACGRBS15400
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50785-ACRRBS15400
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50785-CFRBS13340
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50785-CHRBS13340
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50785-CMRBS13340
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50785-CYRBS13340
Мышь CD208/DC-LAMP Джин клон кДНК в вектор клонированияMG50785-GRBS5130
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50785-NFRBS13340
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50785-NHRBS13340
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50785-NMRBS13340
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50785-NYRBS13340
Мышь CD208/DC-LAMP Джин ORF экспрессии кДНК клона плазмидыMG50785-UTRBS13340
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Фон

Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.

Ссылки
  • Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.
  • Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.
  • Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.
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