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Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse IL36RN Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_019451.1
Размер кДНК:468bp
Описание кДНК:Full length Clone DNA of Mus musculus interleukin 1 family, member 5 (delta) with N terminal Myc tag.
Синоним гена:IL-1H3, IL1HY1, AI413231, FIL1delta
Виды:Mouse
переносчик:pCMV3-SP-N-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка on other vectors
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50213-ACGRBS15400
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50213-ACRRBS15400
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50213-CFRBS13340
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50213-CHRBS13340
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50213-CMRBS13340
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50213-CYRBS13340
Мышь IL-1F5 Джин клон кДНК в вектор клонированияMG50213-MRBS5130
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50213-NFRBS13340
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50213-NHRBS13340
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50213-NMRBS13340
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50213-NYRBS13340
Мышь IL-1F5 Джин ORF экспрессии кДНК клона плазмидыMG50213-UTRBS13340
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Фон

Interleukin-1 family member 5 (IL-1F5), also known as interleukin 36 receptor antagonist (IL36RA), is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to interleukin 1 family member 9 (IL1F9). IL-1F5 could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), which is present in epithelial barriers and takes part in local inflammatory response. It has been proved that IL-1F5 induces IL-4 mRNA and protein expression in glia in vitro and enhances hippocampal expression of IL-4 following intracerebroventricular injection. The inhibitory effect of IL-1F5 on LPS-induced IL-1β is attenuated in cells from IL-4-defective mice. Experiment results suggest that IL-1F5 mediates anti-inflammatory effects through its ability to induce IL-4 production and that this is a consequence of its interaction with the orphan receptor, single Ig IL-1R-related molecule (SIGIRR)/TIR8, as the effects were not observed in SIGIRR−/− mice. In contrast to its effects in brain tissue, IL-1F5 did not attenuate LPS-induced changes, or up-regulated IL-4 in macrophages or dendritic cells, suggesting that the effect is confined to the brain.

Ссылки
  • Nicklin MJ, et al. (1994) A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics. 19 (2): 382-4.
  • Debets R, et al. (2001) Two novel IL-1 family members, IL-1 delta and IL-1 epsilon, function as an antagonist and agonist of NF-kappa B activation through the orphan IL-1 receptor-related protein 2. J Immunol. 167 (3): 1440-6.
  • Costelloe C, et al. (2008) IL-1F5 mediates anti-inflammatory activity in the brain through induction of IL-4 following interaction with SIGIRR/TIR8. J Neurochem. 105(5): 1960-9.
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