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Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse IFNAR1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_010508.2
Размер кДНК:1773bp
Описание кДНК:Full length Clone DNA of Mus musculus interferon (alpha and beta) receptor 1 with N terminal Flag tag.
Синоним гена:Ifar, Ifrc, CD118, Ifnar, Infar, Ifnar1
Виды:Mouse
переносчик:pCMV3-SP-N-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50469-ACGRBS16760
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50469-ACRRBS16760
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50469-CFRBS14710
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50469-CHRBS14710
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50469-CMRBS14710
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50469-CYRBS14710
Мышь IFNAR1 Джин клон кДНК в вектор клонированияMG50469-MRBS5130
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50469-NFRBS14710
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50469-NHRBS14710
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50469-NMRBS14710
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50469-NYRBS14710
Мышь IFNAR1 Джин ORF экспрессии кДНК клона плазмидыMG50469-UTRBS14710
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Фон

Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.

Ссылки
  • Yan H, et al. (1996) Phosphorylated interferon-alpha receptor 1 subunit (IFNaR1) acts as a docking site for the latent form of the 113 kDa STAT2 protein. EMBO J. 15(5): 1064-74.
  • Richter MF, et al. (1998) Specific contribution of Tyk2 JH regions to the binding and the expression of the interferon alpha/beta receptor component IFNAR1. J Biol Chem. 273(38): 24723-9.
  • Abramovich C, et al. (1997) A protein-arginine methyltransferase binds to the intracytoplasmic domain of the IFNAR1 chain in the type I interferon receptor. EMBO J. 16(2): 260-6.
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    Каталог: MG50469-NF
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