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Мышь HAPLN1 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse HAPLN1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:AF139572.1
Размер кДНК:1071bp
Описание кДНК:Full length Clone DNA of Mus musculus hyaluronan and proteoglycan link protein 1.
Синоним гена:LP, CLP, LP-1, Crtl1, Crtl1l, BB099155
Виды:Mouse
переносчик:PGEM-T Vector
Plasmid:pGEM-mHAPLN1
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 804T>C and 987T>G not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Мышь HAPLN1 Джин клон кДНК в вектор клонирования on other vectors
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG52939-ACGRBS15400
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG52939-ACRRBS15400
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаMG52939-ANGRBS15400
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаMG52939-ANRRBS15400
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG52939-CFRBS13340
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG52939-CHRBS13340
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG52939-CMRBS13340
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG52939-CYRBS13340
Мышь HAPLN1 Джин клон кДНК в вектор клонированияMG52939-GRBS5130
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG52939-NFRBS13340
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG52939-NHRBS13340
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG52939-NMRBS13340
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG52939-NYRBS13340
Мышь HAPLN1 Джин ORF экспрессии кДНК клона плазмидыMG52939-UTRBS13340
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Фон

Hyaluronan (HA) is a high MW glycosaminoglycan significantly involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. HAPLN1, also known as CRTL1 (Cartilage Link Protein 1, cLP ) and link protein, is a member of HA-binding protein (hyaladherins) family, and contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. HAPLN1/CRTL1 stabilizes the interaction between hyaluronan (HA) and versican, two extracellular matrix components essential for cardiac development. Link module superfamily can be divided into three subgroups, and the HAPLN family are C domain-type proteins that have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules. In cartilage, aggrecan forms - cLP stabilized aggregates with HA that provides the tissue with its load bearing properties. HAPLN1 is a component of follicular matrix, was shown to enhance cumulus-oocyte complex (COC) expansion in vitro. HAPLN1 may promote periovulatory granulosa cell survival, which would facilitate their differentiation into luteal cells.

Ссылки
  • Sun GW, et al. (2003) Follicle-stimulating hormone and insulin-like growth factor I synergistically induce up-regulation of cartilage link protein (Crtl1) via activation of phosphatidylinositol-dependent kinase/Akt in rat granulosa cells. Endocrinology. 144(3): 793-801.
  • Wirrig EE, et al. (2007) Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development. Dev Biol. 310(2): 291-303.
  • Liu J, et al. (2010) Periovulatory expression of hyaluronan and proteoglycan link protein 1 (Hapln1) in the rat ovary: hormonal regulation and potential function. Mol Endocrinol. 24(6): 1203-17.
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