After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Быстрый заказ

Text Size:AAA

Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse F2 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_010168.2
Размер кДНК:1857bp
Описание кДНК:Full length Clone DNA of Mus musculus coagulation factor II with C terminal Myc tag.
Синоним гена:Cf2, FII, Cf-2, thrombin, F2
Виды:Mouse
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50344-ACGRBS16760
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50344-ACRRBS16760
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50344-CFRBS14710
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50344-CHRBS14710
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50344-CMRBS14710
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50344-CYRBS14710
Мышь Coagulation Factor II/F2 Джин клон кДНК в вектор клонированияMG50344-MRBS5130
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50344-NFRBS14710
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50344-NHRBS14710
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50344-NMRBS14710
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50344-NYRBS14710
Мышь Coagulation Factor II/F2 Джин ORF экспрессии кДНК клона плазмидыMG50344-UTRBS14710
 Узнайте больше о векторов экспрессии,
Product nameProduct name
Фон

Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin / Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin / Coagulation Factor II and factor Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin. Prothrombin / Coagulation Factor II is expressed by the liver and secreted in plasma. Defects in prothrombin / Coagulation Factor II are the cause of factor II deficiency (FA2D). It is very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Defects in Coagulation Factor II are also a cause of susceptibility to thrombosis. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.

Ссылки
  • Danneberg J, et al. (1998) Reliable genotyping of the G-20210-A mutation of Coagulation Factor II (prothrombin). Clin Chem. 44(2): 349-51.
  • Redondo M, et al. (1999) Coagulation Factor s II, V, VII, and X, prothrombin gene 20210GA transition, and factor V Leiden in coronary artery disease: high factor V clotting activity is an independent risk factor for myocardial infarction. Arterioscler Thromb Vasc Biol. 19(4): 1020-5.
  • Miletich JP, et al. (1980) The synthesis of sulfated dextran beads for isolation of human plasma Coagulation Factor s II, IX, and X. Anal Biochem. 105(2): 304-10.
  • All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Внимание: все продукты «ПРЕДНАЗНАЧЕНЫ ИСКЛЮЧИТЕЛЬНО ДЛЯ ИССЛЕДОВАНИЙ И НЕ ПРЕДНАЗНАЧЕНЫ ДЛЯ ДИАГНОСТИКИ ИЛИ ТЕРАПЕВТИЧЕСКОГО ЛЕЧЕНИЯ».