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Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse CTSA Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_001038492.1
Размер кДНК:1425bp
Описание кДНК:Full length Clone DNA of Mus musculus cathepsin A with C terminal Myc tag.
Синоним гена:PPCA, Ppgb, AU019505, Ctsa
Виды:Mouse
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50348-ACGRBS15400
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50348-ACRRBS15400
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50348-CFRBS13340
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50348-CHRBS13340
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50348-CMRBS13340
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50348-CYRBS13340
Мышь Cathepsin A/CTSA Джин клон кДНК в вектор клонированияMG50348-MRBS5130
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50348-NFRBS13340
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50348-NHRBS13340
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50348-NMRBS13340
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50348-NYRBS13340
Мышь Cathepsin A/CTSA Джин ORF экспрессии кДНК клона плазмидыMG50348-UTRBS13340
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Фон

Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.

Ссылки
  • Hiraiwa M. (1999) Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 56(11-12): 894-907.
  • Yoshida T, et al. (2006) Comparative analysis of binding energy of chymostatin with human cathepsin A and its homologous proteins by molecular orbital calculation. J Chem Inf Model. 46(5): 2093-103.
  • Seyrantepe V, et al. (2008) Enzymatic activity of lysosomal carboxypeptidase (cathepsin) A is required for proper elastic fiber formation and inactivation of endothelin-1. Circulation. 117(15): 1973-81.
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