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Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse CNTN3 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_008779.2
Размер кДНК:3087bp
Описание кДНК:Full length Clone DNA of Mus musculus contactin 3 with C terminal Flag tag.
Синоним гена:Pang, Cntn3
Виды:Mouse
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG51007-ACGRBS22240
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG51007-ACRRBS22240
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG51007-CFRBS20190
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG51007-CHRBS20190
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG51007-CMRBS20190
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG51007-CYRBS20190
Мышь Contactin 3/CNTN3 Джин клон кДНК в вектор клонированияMG51007-GRBS5130
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG51007-NFRBS20190
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG51007-NHRBS20190
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG51007-NMRBS20190
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG51007-NYRBS20190
Мышь Contactin 3/CNTN3 Джин ORF экспрессии кДНК клона плазмидыMG51007-UTRBS20190
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Фон

Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.

Ссылки
  • Yoshihara Y, et al. (1994) BIG-1: a new TAG-1/F3-related member of the immunoglobulin superfamily with neurite outgrowth-promoting activity. Neuron 13(2):415-26.
  • Yoshihara Y, et al. (1995) Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily. Journal of neurobiology 28(1):51-69.
  • Yoshihara Y, et al. (1995) Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: four members of an axon-associated cell adhesion molecule subgroup of the immunoglobulin superfamily. Journal of neurobiology 28(1):51-69.
  • Shimoda Y, et al. (2009) Contactins: Emerging key roles in the development and function of the nervous system. Cell adhesion & migration 3(1):64-70.
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    Каталог: MG51007-CF
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