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Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse CD38 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_007646.4
Размер кДНК:915bp
Описание кДНК:Full length Clone DNA of Mus musculus CD38 antigen with N terminal Myc tag.
Синоним гена:Cd38-rs1, Cd38
Виды:Mouse
переносчик:pCMV3-SP-N-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc Метка on other vectors
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50191-ACGRBS15400
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50191-ACRRBS15400
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50191-CFRBS13340
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50191-CHRBS13340
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50191-CMRBS13340
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50191-CYRBS13340
Мышь CD38/ADPRC1 Джин клон кДНК в вектор клонированияMG50191-MRBS5130
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50191-NFRBS13340
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50191-NHRBS13340
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50191-NMRBS13340
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50191-NYRBS13340
Мышь CD38/ADPRC1 Джин ORF экспрессии кДНК клона плазмидыMG50191-UTRBS13340
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Фон

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

Ссылки
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
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    Каталог: MG50191-NM
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    Количество:+-
    Наличие2-3 weeks
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