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Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

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Mouse CD36 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_007643.4
Размер кДНК:1419bp
Описание кДНК:Full length Clone DNA of Mus musculus CD36 antigen with C terminal Flag tag.
Синоним гена:FAT, GPIV, Scarb3, Cd36
Виды:Mouse
переносчик:pCMV3-C-FLAG
Plasmid:pCMV3-mCD36-Flag
Участок рестрикции:KpnI + XbaI (6kb + 1.47kb)
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 6 C>A, 1413 G>T and 1414A>G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Mouse CD36 Gene Plasmid Map
Mouse CD36 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
Mouse CD36 Gene Expression validated Image
Mouse CD36 ORF mammalian expression plasmid, C-Flag tag
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Caption:
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50422-ACGRBS15400
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50422-ACRRBS15400
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаMG50422-ANGRBS15400
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50422-CFRBS13340
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50422-CHRBS13340
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50422-CMRBS13340
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50422-CYRBS13340
Мышь CD36/SCARB3 Джин клон кДНК в вектор клонированияMG50422-MRBS5130
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50422-NFRBS13340
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50422-NHRBS13340
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50422-NMRBS13340
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50422-NYRBS13340
Мышь CD36/SCARB3 Джин ORF экспрессии кДНК клона плазмидыMG50422-UTRBS13340
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Фон

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell types in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.

Ссылки
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Greenwalt RH, et al. (1992) Membrane glycoprotein in CD36: a review of its roles in adherence, signal transduction, and transfusion medicine. The journal of the American society of hematology. 80 (5): 1105-15.
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    • Mouse CD36 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
    • Mouse CD36 ORF mammalian expression plasmid, C-Flag tag
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