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Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse BST1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_008990.2
Размер кДНК:936bp
Описание кДНК:Full length Clone DNA of Mus musculus bone marrow stromal cell antigen 1 with C terminal Myc tag.
Синоним гена:Bp3, BP-3, Ly65, Bsta1, CD157, 114/A10, A530073F09, Bst1
Виды:Mouse
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50319-ACGRBS15400
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50319-ACRRBS15400
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50319-CFRBS13340
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50319-CHRBS13340
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50319-CMRBS13340
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50319-CYRBS13340
Мышь CD157/BST1 Джин клон кДНК в вектор клонированияMG50319-MRBS5130
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50319-NFRBS13340
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50319-NHRBS13340
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50319-NMRBS13340
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50319-NYRBS13340
Мышь CD157/BST1 Джин ORF экспрессии кДНК клона плазмидыMG50319-UTRBS13340
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Фон

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2−) production in the human U937 myeloid line.

Ссылки
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Malavasi F, et al. (2006) CD38 and CD157 as Receptors of the Immune System: A Bridge Between Innate and Adaptive Immunity. Molecular Medicine. 12 (11-12): 334-41.
  • Ortolan E, et al. (2002) CD157, the janus of CD38 but with a unique personality. Cell Biochemistry and Function. 20 (4): 309-22.
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