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Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse BTC Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_007568.4
Размер кДНК:534bp
Описание кДНК:Full length Clone DNA of Mus musculus betacellulin, epidermal growth factor family member with C terminal Myc tag.
Синоним гена:BTC
Виды:Mouse
переносчик:pCMV3-C-Myc
Plasmid:
Участок рестрикции:
Последовательность меток:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Myc Метка on other vectors
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50340-ACGRBS15400
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50340-ACRRBS15400
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50340-CFRBS13340
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50340-CHRBS13340
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50340-CMRBS13340
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50340-CYRBS13340
Мышь Betacellulin / BTC Джин клон кДНК в вектор клонированияMG50340-MRBS5130
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50340-NFRBS13340
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50340-NHRBS13340
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50340-NMRBS13340
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50340-NYRBS13340
Мышь Betacellulin / BTC Джин ORF экспрессии кДНК клона плазмидыMG50340-UTRBS13340
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Фон

Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Ссылки
  • Shing Y, et al. (1993) Betacellulin: a mitogen from pancreatic beta cell tumors. Science . 259(5101): 1604-7.
  • Riese DJ, et al. (1996) Betacellulin activates the epidermal growth factor receptor and erbB-4, and induces cellular response patterns distinct from those stimulated by epidermal growth factor or neuregulin-beta. Oncogene. 12(2): 345-53.
  • Bastian SE, et al. (2001) Measurement of betacellulin levels in bovine serum, colostrum and milk. J Endocrinol . 168: 203-12.
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    Каталог: MG50340-CM
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    Наличие2-3 weeks
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