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Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse B4GALT1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_022305.3
Размер кДНК:1200bp
Описание кДНК:Full length Clone DNA of Mus musculus UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 with N terminal Flag tag.
Синоним гена:GalT, Ggtb, Ggtb2, AA407245, B-1, 4-GalT, B4galt1
Виды:Mouse
переносчик:pCMV3-SP-N-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag Метка on other vectors
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG51056-ACGRBS15396
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG51056-ACRRBS15396
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG51056-CFRBS13343
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG51056-CHRBS13343
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG51056-CMRBS13343
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG51056-CYRBS13343
Мышь B4GALT1/GGTB2 Джин клон кДНК в вектор клонированияMG51056-GRBS5132
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG51056-NFRBS13343
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG51056-NHRBS13343
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG51056-NMRBS13343
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG51056-NYRBS13343
Мышь B4GALT1/GGTB2 Джин ORF экспрессии кДНК клона плазмидыMG51056-UTRBS13343
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Фон

Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.

Ссылки
  • Hennet T. (2002) The galactosyltransferase family. Cellular and Molecular Life Sciences. 59(7): 1081-95.
  • Landers EA, et al. (2009) Porcine 1, 4-Galactosyltransferase-I Sequence and Expression. Reproduction in Domestic Animals. 44(2): 228-34.
  • Amado M, et al. (2000) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1473 (1): 35-53.
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    Каталог: MG51056-NF
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