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Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

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Mouse ALDH7A1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:BC012407.1
Размер кДНК:1536bp
Описание кДНК:Full length Clone DNA of Mus musculus aldehyde dehydrogenase family 7, member A1 with C terminal Flag tag.
Синоним гена:Atq1, D18Wsu181e
Виды:Mouse
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG52196-ACGRBS16760
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG52196-ACRRBS16760
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаMG52196-ANGRBS16760
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаMG52196-ANRRBS16760
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG52196-CFRBS14710
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG52196-CHRBS14710
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG52196-CMRBS14710
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG52196-CYRBS14710
Мышь ALDH7A1 Джин клон кДНК в вектор клонированияMG52196-GRBS5130
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG52196-NFRBS14710
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG52196-NHRBS14710
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG52196-NMRBS14710
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG52196-NYRBS14710
Мышь ALDH7A1 Джин ORF экспрессии кДНК клона плазмидыMG52196-UTRBS14710
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Фон

ALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes.

Ссылки
  • Brocker C, et al. (2011) Aldehyde dehydrogenase 7A1 (ALDH7A1) attenuates reactive aldehyde and oxidative stress induced cytotoxicity. Chem Biol Interact. 191(1-3): 269-77.
  • Brocker C, et al. (2010) Aldehyde dehydrogenase 7A1 (ALDH7A1) is a novel enzyme involved in cellular defense against hyperosmotic stress. J Biol Chem. 285(24): 18452-63.
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    Каталог: MG52196-CF
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