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Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse AKT3 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_011785.3
Размер кДНК:1440bp
Описание кДНК:Full length Clone DNA of Mus musculus thymoma viral proto-oncogene 3 with C terminal Flag tag.
Синоним гена:AI851531, D930002M15Rik, Akt3
Виды:Mouse
переносчик:pCMV3-C-FLAG
Plasmid:
Участок рестрикции:
Последовательность меток:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Описание последовательности:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag Метка on other vectors
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG51027-ACGRBS15400
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG51027-ACRRBS15400
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаMG51027-ANGRBS15400
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаMG51027-ANRRBS15400
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG51027-CFRBS13340
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG51027-CHRBS13340
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG51027-CMRBS13340
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG51027-CYRBS13340
Мышь AKT3 Джин клон кДНК в вектор клонированияMG51027-GRBS5130
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG51027-NFRBS13340
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG51027-NHRBS13340
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG51027-NMRBS13340
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG51027-NYRBS13340
Мышь AKT3 Джин ORF экспрессии кДНК клона плазмидыMG51027-UTRBS13340
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Фон

v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.

Ссылки
  • Mure H, et al. (2010) Akt2 and Akt3 play a pivotal role in malignant gliomas. Neuro Oncol. 12(3): 221-32.
  • Koseoglu S, et al. (2007) AKT1, AKT2 and AKT3-dependent cell survival is cell line-specific and knockdown of all three isoforms selectively induces apoptosis in 20 human tumor cell lines. Cancer Biol Ther. 6(5): 755-62.
  • Cristiano BE, et al. (2006) A specific role for AKT3 in the genesis of ovarian cancer through modulation of G(2)-M phase transition. Cancer Res. 66(24): 11718-25.
  • Robertson GP. (2005) Functional and therapeutic significance of Akt deregulation in malignant melanoma. Cancer Metastasis Rev. 24(2): 273-85.
  • Altomare DA, et al. (2005) Perturbations of the AKT signaling pathway in human cancer. Oncogene. 24: 7455-64.
  • Stahl JM, et al. (2004) Deregulated Akt3 activity promotes development of malignant melanoma. Cancer Res. 64: 7002-10.
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    Каталог: MG51027-CF
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    Наличие2-3 weeks
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