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Мышь AKR1A1 Джин клон кДНК в вектор клонирования

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Mouse AKR1A1 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_021473.3
Размер кДНК:978bp
Описание кДНК:Full length Clone DNA of Mus musculus aldo-keto reductase family 1, member A1 (aldehyde reductase).
Синоним гена:Akr1a4, 2610201A18Rik
Виды:Mouse
переносчик:PGEM-T Vector
Plasmid:pGEM-mAKR1A1
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 636C>T not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Мышь AKR1A1 Джин клон кДНК в вектор клонирования on other vectors
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG52953-ACGRBS15396
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG52953-ACRRBS15396
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, N-GFPSpark МеткаMG52953-ANGRBS15396
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, N-OFPSpark МеткаMG52953-ANRRBS15396
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG52953-CFRBS13343
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG52953-CHRBS13343
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG52953-CMRBS13343
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG52953-CYRBS13343
Мышь AKR1A1 Джин клон кДНК в вектор клонированияMG52953-GRBS5132
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG52953-NFRBS13343
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG52953-NHRBS13343
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG52953-NMRBS13343
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG52953-NYRBS13343
Мышь AKR1A1 Джин ORF экспрессии кДНК клона плазмидыMG52953-UTRBS13343
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Фон

Aldehyde reductase (AKR1A1) is a member of the aldo-keto reductase superfamily, which consists of more than 40 known enzymes and proteins that includes variety of monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase. Aldehyde reductase has wide substrate specificities for carbonyl compounds. These enzymes are implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Aldehyde reductase possess a structure with a beta-alpha-beta fold which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being bound in an extended conformation. This binding is more similar to FAD- than to NAD(P)-binding oxidoreductases. AKR1A1 is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.

Ссылки
  • Bohren KM, et al. (1989) The aldo-keto reductase superfamily. cDNAs and deduced amino acid sequences of human aldehyde and aldose reductases. J Biol Chem. 264 (16): 9547-51.
  • Fujii J, et al. (1999) The structural organization of the human aldehyde reductase gene, AKR1A1, and mapping to chromosome. Cytogenetics and Cell Genetics . 84 (3-4): 33-2.
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    Каталог: MG52953-G
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