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Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, C-His Метка

ПаспортОбзорыСвязанные продуктыПротоколы
Mouse LIGHT/TNFSF14 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_019418.2
Размер кДНК:720bp
Описание кДНК:Full length Clone DNA of Mus musculus tumor necrosis factor (ligand) superfamily, member 14 with His tag.
Синоним гена:LTg, HVEML, LIGHT, Ly113, HVEM-L, Tnfsf14
Виды:Mouse
переносчик:pCMV/hygro-His
Plasmid:pCMV-mTNFSF14-10his
Участок рестрикции:KpnI + XhoI (5.5kb + 0.75kb)
Последовательность меток:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Описание последовательности:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
Mouse LIGHT/TNFSF14 Gene Plasmid Map
Mouse LIGHT / TNFSF14 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged
pCMV/hygro-HIs Vector Information
 
Vector Name pCMV/hygro-His
Vector Size 5687bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV/hygro-His Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаMG50980-ACGRBS15400
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаMG50980-ACRRBS15400
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаMG50980-CFRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаMG50980-CHRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаMG50980-CMRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаMG50980-CYRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин клон кДНК в вектор клонированияMG50980-GRBS5130
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаMG50980-NFRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаMG50980-NHRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаMG50980-NMRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаMG50980-NYRBS13340
Мышь TNFSF14/LIGHT/CD258 Джин ORF экспрессии кДНК клона плазмидыMG50980-UTRBS13340
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Фон

LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / LIGHT / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / LIGHT / CD258 also binds to a soluble nonsignaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 / LIGHT / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4+ and CD8+ T cells. In addition, TNFSF14 / LIGHT / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / LIGHT / CD258 is being actively investigated as a possible basis for cancer treatment.

Ссылки
  • Ogawa T, et al. (2010) CXCR3 binding chemokine and TNFSF14 over expression in bladder urothelium of patients with ulcerative interstitial cystitis. J Urol. 183(3): 1206-12.
  • Kanodia S, et al. (2010) Expression of LIGHT/TNFSF14 combined with vaccination against human papillomavirus Type 16 E7 induces significant tumor regression. Cancer Res. 70(10): 3955-64.
  • Hosokawa Y, et al. (2010) TNFSF14 coordinately enhances CXCL10 and CXCL11 productions from IFN-gamma-stimulated human gingival fibroblasts. Mol Immunol. 47(4): 666-70.
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    • Mouse LIGHT / TNFSF14 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged
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