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Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, N-His Метка

ПаспортОбзорыСвязанные продуктыПротоколы
H5N1 HA Информация о продукте «Клон cDNA»
Gene_bank_ref_id:
Размер кДНК:1707bp
Описание кДНК:Full length Clone DNA of Influenza A H5N1 (Egypt/3300-NAMRU3/2008) Hemagglutinin with N terminal His tag.
Синоним гена:Hemagglutinin, HA
Виды:H5N1
переносчик:pCMV3-SP-N-His
Plasmid:
Участок рестрикции:
Последовательность меток:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Описание последовательности:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ACI06185.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at ambient temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, N-His Метка on other vectors
Грипп А H5N1 (A/Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40049-ACGRBS23610
Грипп А H5N1 (A/Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP МеткаVG40049-ACRRBS23610
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) ORF mammalian expression plasmid (Codon Optimized), C-Flag МеткаVG40049-CFRBS21550
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, C-His МеткаVG40049-CHRBS21550
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, C-Myc МеткаVG40049-CMRBS21550
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, C-HA МеткаVG40049-CYRBS21550
Грипп А H5N1 (A/Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) ORF mammalian expression plasmid (Codon Optimized)VG40049-GRBS7870
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, N-Flag МеткаVG40049-NFRBS21550
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, N-His МеткаVG40049-NHRBS21550
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40049-NMRBS21550
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) (Codon Optimized) ORF mammalian expression plasmid, N-HA МеткаVG40049-NYRBS21550
Грипп А H5N1 (Egypt/3300-NAMRU3/2008) Гемагглютинин(HA) ORF mammalian expression plasmid (Codon Optimized)VG40049-UTRBS21550
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Фон

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Ссылки
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
  • Size / Price
    Каталог: VG40049-NH
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    Количество:+-
    Наличие2-3 weeks
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