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Человек IL-5/IL5 / Interleukin 5 Джин клон кДНК в вектор клонирования

ПаспортОбзорыСвязанные продуктыПротоколы
Human IL5 Информация о продукте «Клон cDNA»
Gene_bank_ref_id:NM_000879.2
Размер кДНК:405bp
Описание кДНК:Full length Clone DNA of Homo sapiens interleukin 5 (colony-stimulating factor, eosinophil).
Синоним гена:EDF, TRF, IL-5
Виды:Human
переносчик:pGEM-T Vector
Plasmid:pGEM-IL5
Участок рестрикции:
Последовательность меток:
Описание последовательности:Identical with the Gene Bank Ref. ID sequence except for the point mutations 276 C/T, 309 A/G and 312 G/A not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Склад:The lyophilized plasmid can be stored at room temperature for three months.
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pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Человек IL-5/IL5 / Interleukin 5 Джин клон кДНК в вектор клонирования on other vectors
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, C-GFPSpark МеткаHG15673-ACGRBS15400
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, C-OFPSpark МеткаHG15673-ACRRBS15400
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, C-Flag МеткаHG15673-CFRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, C-His МеткаHG15673-CHRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, C-Myc МеткаHG15673-CMRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, C-HA МеткаHG15673-CYRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, N-Flag МеткаHG15673-NFRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, N-His МеткаHG15673-NHRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, N-Myc МеткаHG15673-NMRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмиды, N-HA МеткаHG15673-NYRBS13340
Человек IL-5/IL5 / Interleukin 5 Джин ORF экспрессии кДНК клона плазмидыHG15673-UTRBS13340
 Узнайте больше о векторов экспрессии,
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Фон

Interleukin 5 (IL-5) is a member of the interleukin family with length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins / signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered as a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues, and can modulate eosinophil behavior at every stage from maturation to survival.

Ссылки
  • Milburn MV, et al. (1993) A novel dimer configuration revealed by the crystal structure at 2.4 A resolution of human interleukin-5. Nature. 363(6425): 172-176.
  • Lee JS, et al. (1989) The IL-4 and IL-5 genes are closely linked and are part of a cytokine gene cluster on mouse chromosome 11. Somat Cell Mol Genet. 15(2): 143-152.
  • Woodcock JM, et al. (1994) Three residues in the common beta chain of the human GM-CSF, IL-3 and IL-5 receptors are essential for GM-CSF and IL-5 but not IL-3 high affinity binding and interact with Glu21 of GM-CSF. EMBO J. 13 (21): 5176-85.
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    Каталог: HG15673-G
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